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A set of laboratory techniques used for the separation of mixtures in chemistry as well as biology is called Chromatography. The basic concept of separation in chromatography involves passing the mixture to be separated, dissolved in a "mobile phase" through a column or medium called the “stationary phase”, which separates the different components of the mixture. Diversity in any molecule's partition coefficient is responsible for the differential retention levels on the stationary phase thereby causing the separation..

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